Figure 5

DEP modulate LPS-induced MDDC maturation: effect of SR ligands. MDDC were preincubated 2 h with 20 μg/ml dextran sulfate, 100 μg/ml maleylated ovalbumin associated or not with 1 or 10 μg/ml DEP and then, 100 ng/ml LPS were added (Preincubation). For the coincubation, the three ligands are added simultaneously. After 24 h incubation, MDDC were labeled for HLA-DR, CD83 and CD86 and were analyzed by flow cytometry. Data are expressed as the mean ± SEM from 7 independent experiments. ✻: p < 0.05 compared with LPS-treated cells. +: p < 0.05 compared with the condition without SR ligand.