Figure 6

DEP modulate LPS-induced cytokine and chemokine production by MDDC: effect of SR ligands. MDDC were preincubated 2 h with 20 μg/ml dextran sulfate, 100 μg/ml maleylated ovalbumin associated or not with 1 or 10 μg/ml DEP and then, 100 ng/ml LPS were added (Preincubation). For the coincubation, the three ligands are added simultaneously. After 24 h incubation, the concentrations of IP-10, IL-12, IL-6 and TNF-α were measured by ELISA. Data are expressed as the mean ± SEM from 8 independent experiments. ✻: p < 0.05 compared with LPS-treated cells. +: p < 0.05; ++: p < 0.01 compared with the condition without SR ligand.