Figure 8

Effect of DEP on ROS production by MDDC. A. MDDC were incubated in medium alone, with DEP (10 μg/ml) or with PMA (100 ng/ml) for different time periods. ROS production was analyzed by flow cytometry. The left histogram reported the mean ± SEM from 6 independent experiments whereas the flow cytometry histograms show the data obtained in one representative experiment. +: p < 0.05 compared with cells in medium alone. B. Modulation by maleylated ovalbumin of DEP effect on MDDC activated for 2 and 4 h. Data in the left histogram are expressed as the mean ± SEM from 6 independent experiments. The flow cytometry histograms reported the data obtained in one representative experiment. +: p < 0.05 compared with cells in medium alone.