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Figure 3 | Particle and Fibre Toxicology

Figure 3

From: Polycyclic aromatic hydrocarbon components contribute to the mitochondria-antiapoptotic effect of fine particulate matter on human bronchial epithelial cells via the aryl hydrocarbon receptor

Figure 3

Dose-effect studies of the antiapoptotic effect of PM 2.5 -AW. Apoptosis was quantified simultaneously by four parameters and expressed as percentage of 16HBE cells showing ΔΨm drop (DiOC6(3) low) and ROS production (HE->Eth high) or PS exposure (Annexin V+) and plasma membrane permeabilization (PI high). (A and B) After a 4 h pretreatment with PM2.5-AW (10 μg/cm2), 16HBE cells were exposed to the indicated concentrations of A23187. Significance was calculated with respect to medium conditions (*, p < 0.001, n = 3). (C and D) Particle pretreatment was performed with different doses (1-50 μg/cm2) 4 h prior to induction of apoptosis by 3 μM of A23187. Results are means ± S.D (n = 3). Significance was calculated with respect to non-pretreated cells with particles (#, p < 0.001).

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Particle and Fibre Toxicology

ISSN: 1743-8977

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