Figure 1From: Nanomaterial cytotoxicity is composition, size, and cell type dependentNanoparticle mediated protein depletion from cell culture media. Nanoparticles were incubated in serum containing culture media (10%) for periods of 30 minutes and 2 hours, with the early time point representing time for significant protein interaction and the later time point representing onset of particle uptake. Initially, all nanoparticles adsorb approximately 30% of the serum from the media, followed by a period of particle dependent reversible exchange of protein with culture media (a). Plotting of change in protein depletion from the media reveals significant differences in exchange based on nanoparticle properties (b). Vertical lines denote ±1 SD (n = 4 for all tested samples). (*) indicates significant t-test at (P < 0.05). Bracket with a (*) represents significant one-way ANOVA at (P < 0.05).Back to article page