Figure 3

Nanomaterial composition, concentration, and size effects on 3T3, hT and RAW cells. The effect of nanomaterial concentration and composition on survival rates of (a) 3T3 fibroblasts, (b) hT, and (c) RAW cells at 24 hours. Cells were respectively treated with 10, 100, 1000 μg/ml of TiO2, SiO2, MWCNT <8 nm, MWCNT 20-30 nm, and MWCNT >50 nm for 24 hours. Viability was measured using the MTS assay and normalized to untreated cells. Vertical lines denote ±1 SD (n = 4 for all tested samples). Significant ANOVA test among different cell types is represented by bracket with a (*) over each data set. (*) above nanomaterial columns indicates statistically significant difference compared to untreated controls (P < 0.05).