Figure 3

Cell count in bronchoalveolar lavage fluid. The effect of titanium dioxide (TiO₂) inhalation exposure performed for 2 hours a day, three days a week, for four weeks at a concentration of 10 mg/m³ on macrophage, eosinophil, neutrophil and lymphocyte infiltration to bronchoalveolar lavage fluid (BALF) calculated from May-Grünwald-Giemsa (MGG)-stained cytospin slides with light microscopy (x80). Results are shown as cells per high power field (HPF) for healthy (PBS) and allergic (OVA) mice exposed to nanosized (nTiO₂) or fine (fTiO₂) TiO₂ or left unexposed (-). From each slide the cells were counted from three fields from which an average was counted. N = 8 mice per group. The bars represent mean ± SEM; * P < 0.05 and ** P < 0.01 significantly different from unexposed control; Mann-Whitney U test.