Figure 6

DEP-induced phosphorylation of p38 (A), phosphorylation of p65 (B) and degradation of IκBα (C). Human bronchial epithelial BEAS-2B cells were exposed to increasing concentrations of DEPs (0, 100, 200 μg/ml) for 2 or 4 h before Western analysis. Each figure displays a representative blot and optical quantification of the protein bands from separate replicate experiments. Detected levels of phosphorylated p38 and p65 and the levels of IκBα are respectively normalised against total p38, p65, or β-actin, and are presented as fold increase (mean ± SEM, n≥3) compared to unexposed cells.