Figure 8

Effects of siRNA against NF-κB (p65) on DEP-induced mRNA expression of genes. Effects of siRNA against NF-κB (p65) on DEP-induced expression of IL-6 (A), IL-8 (B), COX-2 (C) and CYP1A1 (D) mRNA. Successful transfection was verified by Western analysis of p65-levels (E). Human bronchial epithelial BEAS-2B cells were exposed to DEPs (0 and 100 μg/ml) for 4 h. Relative quantification of cytokine mRNA levels was performed by QRT-PCR. Bars represent means ± SEM of fold increase relative to unexposed cells detected in separate experiments (n = 4). * p < 0.05 control vs. p65 siRNA + (targeted).