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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: Particulate matter air pollution causes oxidant-mediated increase in gut permeability in mice

Figure 2

The generation of mitochondrial ROS is required for PM-induced cell death in colonic epithelial cells. (A) ROS production and (B) cell death were measured 4 and 24 hours, respectively, after treatment of Caco-2 cells stably expressing mitochondrial oxidant sensitive GFP (mito-Ro-GFP) with control (media) or PM in the absence or presence of the combined superoxide dismutase and catalase mimetic, EUK -134 (20 μM). (C) ROS production and (D) cell death were measured at 4 and 24 hours, respectively, after treatment of wild-type Caco-2 cells and Caco-2 cells lacking mitochondrial DNA (ρ0 CaCo-2 cells) with vehicle (media) or PM. The t-butyl hydroperoxide (t-BOOH) was used as a positive control. (P < 0.05 *PM compared with PBS, **EUK-134 compared with vehicle, n ≥ 4).

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