Figure 5
From: Particulate matter air pollution causes oxidant-mediated increase in gut permeability in mice
Exposure of monolayers of Caco-2 cells to PM causes increased permeability and disruption of tight junctions. Caco-2 cells were treated with either PBS (vehicle) or different concentrations of PM and we (A) stained the cell monolayers for ZO-1, a tight junction protein and (B) measured mean tight junction disruption Intestinal sections from the animals were subjected to a blinded analysis of tight junction disruption. Representative images from 4 independent experiments are shown and each bar represents the mean of 20 observations.