Figure 1
From: Darkfield-Confocal Microscopy detection of nanoscale particle internalization by human lung cells
Schematic diagram of an inverted confocal system equipped for simultaneous fluorescence and darkfield imaging. In this example, source laser light (Blue) is focused on the specimen for fluorescence excitation. Fluorescence light emitted by the specimen (Green) is collected by the objective in a conventional CLSM manner. The darkfield condenser collects the scattered light. The numerical aperture of the objective is adjusted using the iris diaphragm to allow only excitation source light that is scattered by the specimen to reach the transmitted light detector (TD).