Figure 3
From: Darkfield-Confocal Microscopy detection of nanoscale particle internalization by human lung cells
Darkfield(DF)/Confocal(CLSM) imaging of TiO 2 nano particles internalized by human bronchial epithelial cells. BEAS 2B cells were exposed to 27 nm TiO2 for 2 hours followed by fixation and staining with HCS Cell Mask-Blue to visualize the cytoplasm. Shown is a pseudo-colored side view taken along the Z-axis of these cells. Particles appear as elongated structures due to the lack of confocality in the darkfield optical path. The inset displays a top (XY) view of the same cells. Cells were excited using only the 404 laser line while fluorescence was monitored using the blue channel. Magnification 1800x with a 3X zoom. 60x plan fluor objective