Figure 5
From: Darkfield-Confocal Microscopy detection of nanoscale particle internalization by human lung cells
Determination of nanoparticle location using the maximum intensity technique. Scatter and fluorescence intensity plots through the z-axis of the entire cell volume are plotted for cells incubated with TiO2 for 5 min and 2 hr time points for both a selected particle of interest (circled in red) and the corresponding nucleus, respectively. Fluorescent and scatter light intensity values have been normalized to the same peak height for clarity. Scale bar represents 10 μm. Magnification 3000x with a 5X zoom.