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Figure 7 | Particle and Fibre Toxicology

Figure 7

From: Regulation of the arachidonic acid mobilization in macrophages by combustion-derived particles

Figure 7

The water-insoluble fraction of MAF02 is responsible for AA mobilization and activation of ERK1/2 and c-Jun. For analysis of ERK1/2 (A), and c-Jun (D) phosphorylation non-labelled cells were exposed with 50 μg/ml (15.6 μg/m2) of the total, water-soluble and water-insoluble fraction of fly ash particles for 2.5 hours. Whole cell lysates were analyzed by Western blotting. OD-band intensities of phosphorylated proteins, analyzed by Odyssey® software, were normalized to the respective loading control protein and expressed in relation to the maximum band intensity of the MAF02-treated sample (100%). (B, C) RAW264.7 cells labelled with [14C]arachidonic acid were treated with 50 μg/ml (13.2 μg/m2) of the total, water-insoluble and water-soluble fraction of fly ash particles for 2.5 hours. After lipid extraction, the free arachidonic acid and its metabolites were separated by TLC, visualized by autoradiography and analyzed by OptiQuant® and Odyssey®software. Data on AA liberation are expressed as percentage of control cells (100% values). Results are presented as the mean ± s.e.m. of three independent experiments.

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