Figure 2
Endothelium-dependent acetylcholine (ACh)-induced vasodilation of aorta segments from ApoE-/- mice exposed by intratracheal instillation to control solution containing 90% saline and 10% BAL fluid (black), nTiO 2 (green), fTiO 2 (blue), or pTiO 2 (red). The measurements are performed in A) absence or B) ex vivo presence of the SOD mimic, tempol. The response is expressed as the percent vasodilation of the pre-contraction tension produced by PGF2α. Each point on the curves represents the cumulative response at each concentration of the vasodilator. Data are expressed as means ± SEM (n = 10-11). * P < 0.05 (ANOVA) compared with Emax in segments that were not treated with tempol. # P < 0.05 (ANOVA) compared with Emax values in tempol-treated aorta segments of control mice.