Figure 5

Transmembrane electrical resistance was measured for cocultures of H441 with ISO-HAS-1 (H441/ISO-HAS-1) as well as for cocultures of primary isolated cells (alveolar type II and HPMEC (ATII/HPMEC)). During 4 h incubation with aSNPs (NexSil20, Ludox TM-40 at concentrations of 6, 60, 600, and 6000, μg/ml) TER-values are depicted as % of time-point t0 (TER-value prior to aSNP treatment). Results are shown as means ± S.D. of 3 independent experiments with n = 2 samples for each treatment. For statistical analysis using Dunnett's Multiple Analyzing test, the 4 h value of the untreated samples was used as control. Treatment with 600 and 6000 μg/ml of both aSNP revealed a time-dependent decrease of TER after 4 h incubation. *P < 0.05, ** P < 0.01 and *** P < 0.001 compared to the untreated control