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Figure 6 | Particle and Fibre Toxicology

Figure 6

From: Inflammatory and cytotoxic responses of an alveolar-capillary coculture model to silica nanoparticles: Comparison with conventional monocultures

Figure 6

After aSNP exposure layer integrity of H441 and ISO-HAS-1 in coculture was determined by immunofluorescent localization of junction-associated proteins. The H441 cells of the coculture were incubated with aSNP (NexSil20: concentration range 0.6 - 6000 μg/ml, c: untreated control) for 4 h in serum-free medium. aSNPs were then removed and cells were cultivated for further 20 h. The H441 were labelled for E-cadherin, the ISO-HAS-1 were counterstained for PECAM-1.

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