Figure 7

Protein expression of cell death regulators was analysed after apical exposure of differentiated cocultures with aSNPs (NexSil20: 600 μg/ml) to mimic the situation after accidental inhalation of aSNPs. After 4 h incubation with aSNPs filter membranes were excised, lysed for 30 min and an apoptosis protein array was performed. Data are depicted as means ± S.D. from 2 independent experiments with n = 4 samples for each treatment. Quantitative analysis of the array revealed increased levels of a number of apoptosis markers, especially of the intrinsic pathway.