Figure 3
Soft X-ray microscopy and XRF analyses of an asbestos body in human lung tissue. Panel A: visible light image of the tissue with the asbestos body. Panels B and C: absorption and phase contrast images respectively acquired at a photon energy of 2018 eV (25 × 40 μm2 size, 30 ms dwell time, 415 nm spot size). Panels Fe, Si and Mg: elemental distribution (25 × 35 μm2 size, 30 s/pixel dwell time, 1 μm spot size) among the area of panels B and C, acquired at 2 keV, (Si and Mg), and at 1,3 keV (Fe). Panel D and E: XRF spectra collected in the points a and b (see panel B) at 1.3 keV (D) and at 2 keV (E) with 180 s acquisition time).