Figure 5
Production of inflammation-related soluble factors by CaCo-2 cells. Cells were chronically exposed for 15 days to culture medium alone or containing IL-1β (10 ng/ml, positive control), Au NPs 4 nm (5.2 μg/ml), CoO NPs (3.5 μg/ml), CeO2 NPs (1.3 μg/ml) (all corresponding to 9.1% v/v), or their solvents. Factors were detected in the cell supernatants by Proteome Profiler Antibody Array and evaluated as arbitrary units by densitometric analysis. Results for IL-1β, sICAM-1, CXCL1 and CXCL4 are reported in the figure, and presented as mean values ± SD from 2-4 replicate determinations.