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Figure 5 | Particle and Fibre Toxicology

Figure 5

From: Problems and challenges in the development and validation of human cell-based assays to determine nanoparticle-induced immunomodulatory effects

Figure 5

Production of inflammation-related soluble factors by CaCo-2 cells. Cells were chronically exposed for 15 days to culture medium alone or containing IL-1β (10 ng/ml, positive control), Au NPs 4 nm (5.2 μg/ml), CoO NPs (3.5 μg/ml), CeO2 NPs (1.3 μg/ml) (all corresponding to 9.1% v/v), or their solvents. Factors were detected in the cell supernatants by Proteome Profiler Antibody Array and evaluated as arbitrary units by densitometric analysis. Results for IL-1β, sICAM-1, CXCL1 and CXCL4 are reported in the figure, and presented as mean values ± SD from 2-4 replicate determinations.

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