| | Gene expression (AU)
b
|
---|
Treatment
a
|
IL-18
|
IL-18Rα
|
| |
no NPs
|
AgO NPs
|
no NPs
|
AgO NPs
|
4 h | medium | 0.77 ± 0.40 | 0.98 ± 0.20 n.s.
| 0.49 ± 0.04 | 0.47 ± 0.13 n.s.
|
| endotoxin | 3.57 ± 0.80 | 3.04 ± 0.03 n.s.
| 0.65 ± 0.16 | 1.78 ± 0.37* |
24 h | medium | 0.92 ± 0.11 | 0.92 ± 0.25 n.s.
| 0.27 ± 0.12 | 0.82 ± 0.56 n.s.
|
| endotoxin | 0.31 ± 0.05 | 0.17 ± 0.05 n.s.
| 18.56 ± 1.86 | 10.17 ± 2.01* |
-
a Human monocytes were exposed for 4 and 24 h to culture medium alone or to medium containing 50 EU/ml endotoxin. At the beginning of the incubation, cells were exposed to endotoxin-free AgO NPs (4.9 μg/ml, corresponding to a dilution at 4.55% v/v). Controls were cells exposed to culture medium alone or to a 4.55% dilution of the solvent (no detectable difference).
-
b Gene expression was assessed by real-time PCR, using β-actin as housekeeping gene. Data are mean ± SD of replicate experiments.
- * p < 0.05 vs. corresponding treatment in the absence of NPs; n.s. not significant vs. corresponding treatment in the absence of NPs