a Human monocytes (3-5 × 106 cells/well) were exposed for 24 h to culture medium alone or to 0.6-4.9 μg/ml (corresponding to 4.55% v/v) of different NPs or their solvents.
b RNA recovery in control cells varied between 700 and 1400 ng/106 cells in seven separate experiments. Recovery in NP-treated cells is calculated as percent of the recovery in control cells (exposed to culture medium alone) within each single experiment, and expressed as mean ± SD. Recovery in cells treated with AgO NPs, tested in four experiments, was highly variable.
c A value of RIN (RNA Integrity Number) between 7 and 10 indicates high quality intact RNA.