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Table 3 RNA recovery and integrity from human monocytes exposed to NPs

From: Problems and challenges in the development and validation of human cell-based assays to determine nanoparticle-induced immunomodulatory effects

Treatment a RNA recovery (% control ± SD) b RNA integrity (RIN) c
Medium 100.0 9.6
Au NPs 4 nm 96.3 ± 5.8 9.6
AgO NPs 78.8 ± 7.7 9.5
CoO NPs 97.2 ± 3.8 9.6
CeO 2 NPs 56.5 ± 4.9 * 9.8
Solvents (all) 97.3 ± 3.4 9.5
  1. a Human monocytes (3-5 × 106 cells/well) were exposed for 24 h to culture medium alone or to 0.6-4.9 μg/ml (corresponding to 4.55% v/v) of different NPs or their solvents.
  2. b RNA recovery in control cells varied between 700 and 1400 ng/106 cells in seven separate experiments. Recovery in NP-treated cells is calculated as percent of the recovery in control cells (exposed to culture medium alone) within each single experiment, and expressed as mean ± SD. Recovery in cells treated with AgO NPs, tested in four experiments, was highly variable.
  3. c A value of RIN (RNA Integrity Number) between 7 and 10 indicates high quality intact RNA.
  4. * p < 0.05 vs. medium control