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Table 2 Interference of nanomaterials with in vitro toxicity test systems

From: Cytotoxicity screening of 23 engineered nanomaterials using a test matrix of ten cell lines and three different assays

  Parameters and particle condition
  Oxidative stress Metabolic activity Cell death
  DCF fluorescence MTT light absorption INT light absorption
  Particle dispersion on cells
[μg cm -2 ]
Particles on cells after washing
[μg cm -2 ]
Particle dispersion on cells
[μg cm -2 ]
Particles on cells after washing
[μg cm -2 ]
Particle dispersions cell free
[μg cm -2 ]
Particles 10 50 10 50 10 50 10 50 10 50
AlOOH I + + - + + + - + - +
AlOOH II + + - + + + - + - +
Al-Ti-Zr 1 + + - + + + - + - +
Al-Ti-Zr 2 + + - + + + - + - +
Al-Ti-Zr 3 + + - + + + - + - +
BaSO 4 + + - + + + - + - +
Carbon Black + + (+) + + + + + - +
CeO 2 + + - + + + - + - +
CeO 2 A + + - + + + - + - +
CeO 2 B + + - + + + - + - +
CeO 2 C + + - + + + - + - +
CeO 2 D + + - + + + - + - +
SrCO 3 1 + + - + + + - + - +
SrCO 3 2 + + - + + + - + - +
TiO 2 2 + + - + + + - + - +
TiO 2 1 + + - + + + - + - +
TiO 2 3 + + - + + + - + - +
Ti-Zr 1 + + - + + + - + - +
Ti-Zr 2 + + - + + + - + - +
Ti-Zr 3 + + - + + + - + - +
ZrO 2 1 + + - + + + - + - +
ZrO 2 2 + + - + + + - + - +
ZrO 2 3 + + - + + + - + - +
  1. optical interference. - no interference detected; (+) slight interference detected; + interference detected