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Figure 4 | Particle and Fibre Toxicology

Figure 4

From: Multi-walled carbon nanotubes induce COX-2 and iNOS expression via MAP Kinase-dependent and -independent mechanisms in mouse RAW264.7 macrophages

Figure 4

Activation of ERK1,2 in RAW264.7 cells by MWCNTs. A) Western blot analysis using an antibody specific for phosphorylated ERK (p-ERK1,2) showing that MWCNTs (50 μg/ml) increased ERK activation in a concentration dependent manner in RAW 264.7 cells. CBNPs did not cause a consistent concentration-dependent activation of ERK. Total levels of ERK1,2 protein and β-actin were not changed by MWCNTs or CBNPs. B) Western blot analysis showing time course of ERK phosphorylation by MWCNTs. Numeric values above each Western blot represent the fold-increase in relative densitometric intensity of each p-ERK1,2 band relative to control (zero) nanoparticle treatment which was normalized to 1.0.

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