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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: A mixture of anatase and rutile TiO2 nanoparticles induces histamine secretion in mast cells

Figure 2

Measurement of the [Ca2+ ] C and calcein leakage after TiO 2 NP exposure. (A) RBL-2H3 cells were stimulated with TiO2 NPs with concentrations of 0.1 mg/ml (orange), 0.25 mg/ml (green), 0.5 mg/ml (purple), 0.75 mg/ml (blue) and 1 mg/ml (red) in normal Hanks' solution, (B) in Ca2+-free Hanks' solution, (C) in the presence of CdCl2 (200 μM), (D) nifedipine (10 μM), (E) verapamil (100 μM), and (F) calcein (50 μM) (n ≥ 12, **p < 0.005) (all colors are as depicted in Figure 2A and each line is a representative fluorescence intensity of approximately 200 cells).

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