Figure 7From: Type I interferon and pattern recognition receptor signaling following particulate matter inhalation Effects of multiple different pulmonary exposures on lung mRNA levels of Irf7 and associated pattern recognition receptors. Lung RNA extracted from previously published exposures was assessed post-hoc with respect to the new findings from this study. Male C57BL/6 J mice were exposed to 340 μg of gas metal arc – stainless steel (GMA-SS), 340 μg gas metal arc – mild steel (GMA-MS), 40 μg of multi-walled carbon nanotubes (MWCNT), or 40 μg single-walled carbon nanotubes (SWCNT) (n = 5 or 6 per group for all exposures) by pharyngeal aspiration and lungs were harvested 24 hr post-exposure [15, 19]. Relative mRNA levels of Irf7 (interferon regulatory factor 7) and the pattern recognition receptors Ddx58 (DEAD box polypeptide 58; RIG-I) and Ifih1 (interferon induced with helicase C domain; MDA5) were increased in the lungs of GMA-SS exposed mice only in reference to sham (dotted line). *p<0.05 vs sham.Back to article page