Skip to main content
Figure 4 | Particle and Fibre Toxicology

Figure 4

From: Action of polystyrene nanoparticles of different sizes on lysosomal function and integrity

Figure 4

EAhy 926 cells stained with acridine orange after treatment with DMEM + 2%FBS (a), 25 μM chloroquine (positive control, b), 20 μg/ml 20 nm CPS (c) and 20 μg/ml 200 nm CPS (d). DNA and RNA are stained green by acridine orange; healthy lysosomes stain only red. Upon exposure to chloroquine (b) lysosomes increase in size and stain in green and red (yellow, arrow head) indicating increase in lysosomal pH and lysosomal swelling. Cells treated with CPS (c, d) show red lysosomes and only rarely double-stained yellow lysosomes (arrows). Scale bar: 10 μm

Back to article page