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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: Glutathione-S-transferase M1 regulation of diesel exhaust particle-induced pro-inflammatory mediator expression in normal human bronchial epithelial cells

Figure 2

GSTM1 knockdown enhanced DEP-induced IL-8 and IL-1β expression in HBEC. A and B, HBEC were infected with lentiviral scrambled or GSTM1 shRNA particles (10 moi) for 24 h, respectively. The cells were lysed for GSTM1 mRNA measurement using RT-PCR (*P < 0.01)or GSTM1 protein determination using immunoblotting. Infected HBEC with lentiviral scrambled or GSTM1 shRNA particles were treated with PBS control or 50 μg/ml DEP for 24 h. Levels of IL-8 (C) and IL-1β (D) proteins in the supernatant of culture media were determined using ELISA and expressed as fold over control (DEP vs. PBS control in scrambled or GSTM1 shRNA group, respectively). *P < 0.05, compared to DEP treatment in the scrambled shRNA group.

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