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Figure 5 | Particle and Fibre Toxicology

Figure 5

From: Glutathione-S-transferase M1 regulation of diesel exhaust particle-induced pro-inflammatory mediator expression in normal human bronchial epithelial cells

Figure 5

GSTM1 knockdown enhances DEP-induced ROS production. A, HBEC were treated with 50 μg/ml DEP for 1 h. Intracellular ROS levels were measured using the fluorescent probe carboxy-H2DCFDA and flow cytometry. B, HBEC were infected with 10 moi of lentiviral non-target (scrambled) or GSTM1 shRNA particles for 24 h, respectively. Confluent cells were treated with 50 μg/ml DEP for 4 h and ROS levels measured as described previously. *, compared to PBS control (Ct), P < 0.05.

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