Figure 6

NAC reduced DEP-induced ERK and Akt phosphorylation, and IL-8 and IL-1β expression. A and B, HBEC were pretreated with 10 mM NAC for 2 h before further stimulation with 50 μg/ml DEP for 1 h. Phosphorylated ERK and Akt was measured using imunoblotting. C and D, HBEC were pretreated with 10 mM NAC for 2 h before further stimulation with 50 μg/ml DEP for 24 h. Levels of IL-8 and IL-1β proteins were measured using ELISA. *, compared to DEP, P < 0.05.