Figure 3

IL-1β release induced by non-crystalline and crystalline silica particles in primary rat lung macrophages. The cells were primed for 3 h with and without LPS (25 ng/ml) and subsequent exposed to different non-crystalline silica particles and MinUsil 5 (MinU) for 6 h. A) Effects of the 200 μg/ml Si50 and Si500, and 50 μg/ml fumed silica (fumed Si), fused silica (fused Si) and MinU in LPS-primed cells B) Effects with or without LPS and subsequent exposure to Si50 (200 μg/ml) and MinU (50 μg/ml) C) Concentration (mass/ml)-response relationships for Si50 versus Si500 on IL-1β release in LPS-primed cells D) Concentration (mass/ml)-response relationships for fumed- and fused silica and MinU on IL-1β release in LPS-primed cells. Fused silica particles after wet sedimentation were used. E) IL-1β responses in relation to particle surface areas in LPS-primed cells. In E the results for Si50, Si500 and fumed silica particles, besides MinUsil 5, are presented. The IL-1β levels were determined by ELISA. In A) the variability of the results is presented by the mean ± SD, including data from 5 experiments. * = p < 0.05 versus control, using Kruskal-Wallis test with Dunn’s Multiple Comparison test as a post-test. In B- E representative experiments are given, all performed in duplicates as indicated.