Figure 5
From: Comparison of non-crystalline silica nanoparticles in IL-1β release from macrophages
Non-crystalline and crystalline silica particles induce IL-1β release in RAW264.7 macrophages via the NALP3 inflammasome. A) The cells were transfected with siRNA against NALP3 as well a negative control for 48 h, then primed for 3 h by LPS (25 ng/ml before exposure to Si50 (200 μg/ml) or MinUSil 5 (200 μg/ml). The IL-1β levels were determined by ELISA. B) The NALP3 mRNA levels after transfection by siRNA against NALP3 and by a negative control siRNA. The NALP3 mRNA levels were adjusted against the respective actin-mRNA levels. Data presented are representative of two experiments performed in duplicates.