Figure 5
Fibre-length dependent impaired migration of BMMs in a wound-healing assay. A,B) An artificial wound was created in the BMM monolayer using a pipette tip and the migration of BMMs into the wound was assessed after treatment with VC, AgP, AgNW3, AgNW5, AgNW14, AgNW28. The dose was adjusted to fibre number and based on 2.5 μg/cm2 for AgNW14. Photographs were taken immediately and at 30 hour after creating the wound. For BSE images were taken at the edge of the wound. No impairment of cell adhesion and spreading could be observed and in fact increased cell spreading due to uptake of longer fibres was observed. Nanoparticles and fibres could be visualised underneath the cell membrane as indicated by the white arrow in the inserts which are the non-overlayed BSE images. Representative images are shown; similar results are obtained in 3 independent experiments.