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Figure 6 | Particle and Fibre Toxicology

Figure 6

From: Use of silver nanowires to determine thresholds for fibre length-dependent pulmonary inflammation and inhibition of macrophage migration in vitro

Figure 6

Inhibition of BMMs migration in a wound-healing assay and cell viability. A) The number of cell migrated was counted and expressed as percentage of cells migrated into the wound normalised to VC. Significant reduction of migration was seen with AgNW5, AgNW14, AgNW28 and cytochalasin D, a positive control for actin cytoskeleton disruption. B) Metabolic activity of BMMs was assessed via the reduction of AlamarBlue® at an absorbance of 570 nm. At the dose used no significant reduction in metabolic activity was measured. C) The integrity of the cell membrane after the different treatments was measured via the release of LDH into the cell supernatant and compared to VC and TX, positive control. A sub-lethal dose was chosen at which none of the treatments lead to a significant increase in cell permeability. Significance indicated compares treatment groups to vehicle control, * indicates p < 0.05, ** indicates p < 0.001, *** indicates p < 0.0001 (n = 3). Data represent mean ± SEM of n = 3.

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