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Figure 1 | Particle and Fibre Toxicology

Figure 1

From: Carbon nanoparticles induce ceramide- and lipid raft-dependent signalling in lung epithelial cells: a target for a preventive strategy against environmentally-induced lung inflammation

Figure 1

CNP trigger lipid changes in rafts and subsequent EGFR and SFK activation. RLE-6TN cells were incubated with CNP [10 μg/cm2, 5 min] or the indicated doses of C6 ceramide [μM, 15 min] respectively. (A) Amount of ceramide, GM3, sphingomyelin, and cholesterol was detected after HPTLC. (B) Phosphorylation of EGFR at Tyr1173, (C + D) SFK at Tyr416, and (E) ERK1/2 determined by Western Blot using phospho-specific antibodies. Equal loading was confirmed with GAPDH or total EGFR. C32, compound 32, * significantly different from respective control, † significantly different from CNP-treatments without C32 pre-treatment [10 μM, 1 h].

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Particle and Fibre Toxicology

ISSN: 1743-8977

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