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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: Carbon nanoparticles induce ceramide- and lipid raft-dependent signalling in lung epithelial cells: a target for a preventive strategy against environmentally-induced lung inflammation

Figure 2

CNP induce loss of SFK and EGFR from lipid rafts. (A) RLE-6TN cells were treated with CNP [10 μg/cm2, 5 min], C6 [5 μM, 15 min], or CP [10 μg/cm2, 5 min] and raft and non-raft fractions were isolated and pooled according to the scheme depicted in the supplementary material. Western Blot analyses using total SFK or EGFR antibodies were performed. *, Significantly different to PBS control (p < 0.05). (B) RLE-6TN cells were treated with CNP [10 μg/cm2, 5 min], CP [10 μg/cm2, 5 min], ectoine [1 mM, 4 h pre-treatment] + CNP, or C6 [5 μM, 15 min]. EGFR (red) localization was analyzed by immunostaining and fluorescence microscopy. Nuclei were stained with DAPI (blue).

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Particle and Fibre Toxicology

ISSN: 1743-8977

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