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Table 2 Mean ± SEM of gene expression (mRNA levels per 106 18 S mRNA) and the geometric mean ± SEM of adhesion molecule expression by FACS analysis in peripheral blood mononuclear cells (PBMCs) at four times after each of the three exposure scenarios

From: Controlled human wood smoke exposure: oxidative stress, inflammation and microvascular function

  

Clean air

  

Low level wood smoke exposure

High level wood smoke exposure

 

Before

0 h

6 h

20 h

Before

0 h

6 h

20 h

Before

0 h

6 h

20 h

Gene expression

           

CCL2

0.08 ± 0.04

0.05 ± 0.02

0.06 ± 0.02

0.10 ± 0.05

0.15 ± 0.09

0.18 ± 0.10

0.12 ± 0.05

0.02 ± 0.02

0.06 ± 0.02

0.14 ± 0.09

0.16 ± 0.08

0.22 ± 0.11

ITGAL

52.9 ± 22.5

57.0 ± 28.4

70.2 ± 33.6

173 ± 108

206 ± 172

203 ± 135

81.3 ± 36.4

15.8 ± 3.99

72.7 ± 37.6

46.8 ± 22.9

49.6 ± 17.0

86.01 ± 51.7

TNF

75.1 ± 73.0

4.10 ± 1.64

4.31 ± 1.48

8.14 ± 2.86

7.43 ± 3.46

8.91 ± 3.74

4.78 ± 1.49

2.35 ± 1.60

3.61 ± 1.63

2.42 ± 0.99

59.0 ± 57.2

68.7 ± 66.3

IL6

0.07 ± 0.06

0.03 ± 0.01

0.05 ± 0.03

0.08 ± 0.05

0.14 ± 0.10

0.24 ± 0.15

0.15 ± 0.09

0.01 ± 0.00

0.05 ± 0.02

0.03 ± 0.01

0.11 ± 0.09

0.42 ± 0.38

IL8

0.11 ± 0.06

0.03 ± 0.01

0.03 ± 0.01

0.05 ± 0.02

0.09 ± 0.04

0.19 ± 0.10

0.14 ± 0.09

0.02 ± 0.01

0.06 ± 0.02

0.09 ± 0.06

0.07 ± 0.04

0.06 ± 0.03

HMOX1

2.13 ± 0.54

4.69 ± 2.01

3.86 ± 1.48

5.00 ± 1.94

4.36 ± 1.97

6.17 ± 2.65

4.07 ± 1.24

1.12 ± 0.28

7.67 ± 4.72

3.41 ± 1.15

2.95 ± 0.56

2.76 ± 0.77

OGG1

3.69 ± 2.37

2.47 ± 1.44

2.56 ± 1.33

3.28 ± 1.67

2.82. ± 1.54

5.17 ± 2.43

4.81 ± 1.78

1.85 ± 1.14

3.35 ± 2.08

2.65 ± 1.06

6.33 ± 3.30

6.31 ± 14.3

Adhesion molecules

          

ICAM1

122 ± 3.34

123 ± 3.16

118 ± 3.40

120 ± 4.26

122 ± 15.6

119 ± 2.11

124 ± 2.56

124 ± 2.45

117 ± 2.72

122 ± 3.84

121 ± 2.82

123 ± 3.03

ITGAL

114 ± 9.13

117 ± 9.60

104 ± 16.5

130 ± 4.26

121 ± 3.49

109 ± 9.19

120 ± 9.16

111 ± 9.20

114 ± 10.8

105 ± 9.63

115 ± 10.5

123 ± 11.8

L-Selectin

227 ± 24.3

146 ± 11.4

159 ± 16.5

157 ± 11.9

234 ± 17.9

196 ± 15.1

202 ± 14.0

262 ± 31.8

223 ± 28.3

205 ± 19.4

189 ± 18.5

155 ± 13.5

  1. p > 0.05 for all differences