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Table 3 Ex vivo contractile and relaxant responses in arterial rings isolated 6 and 24 h after instillation of rats with diesel exhaust particles (DEP) or saline

From: Diesel exhaust particulate induces pulmonary and systemic inflammation in rats without impairing endothelial function ex vivo or in vivo

  phenylephrine acetylcholine sodium nitroprusside isoprenaline
  logEC 50 Max
(% response to KCl)
logIC 50 Imax
(% preconstriction)
logIC 50 Imax
(% preconstriction)
logIC 50 Imax
(% preconstriction)
Aorta         
6 h         
saline -7.24 ± 0.14 67.1 ± 8.1 -7.88 ± 0.20 94.9 ± 1.3 -8.67 ± 0.13 96.7 ± 1.0 -7.52 ± 0.15 90.0 ± 1.8
DEP -7.03 ± 0.07 88.6 ± 5.1* -7.75 ± 0.11 96.1 ± 1.4 -8.70 ± 0.11 87.9 ± 1.0 -8.07 ± 0.10* 93.1 ± 2.2
24 h         
saline -7.29 ± 0.13 120.8 ± 14.5 -7.52 ± 0.10 99.3 ± 0.5 -8.43 ± 0.13 99.3 ± 0.6 -7.62 ± 0.16 88.0 ± 5.8
DEP -7.38 ± 0.11 115.3 ± 13.0 -7.67 ± 0.17 96.2 ± 1.5 -8.60 ± 0.10 98.9 ± 0.4 -7.62 ± 0.26 87.8 ± 6.4
Femoral Artery         
6 h         
saline -5.59 ± 0.08 115.1 ± 24.2 -6.69 ± 0.11 68.9 ± 4.9 -7.80 ± 0.15 94.1 ± 2.9 -6.39 ± 0.29 67.3 ± 7.1
DEP -5.53 ± 0.06 91.8 ± 4.0 -6.81 ± 0.14 77.2 ± 5.6 -7.81 ± 0.18 95.1 ± 1.9 -6.51 ± 0.27 77.4 ± 3.9
24 h         
saline -5.46 ± 0.24 136.0 ± 32.4 -6.79 ± 0.18 61.2 ± 4.7 -7.64 ± 0.18 92.7 ± 2.7 -7.03 ± 0.14 41.8 ± 4.8
DEP -5.46 ± 0.09 124.2 ± 11.4 -6.86 ± 0.14 68.3 ± 6.3 -7.81 ± 0.18 92.5 ± 2.2 -7.07 ± 0.23 58.7 ± 6.5
Mesenteric Artery         
6 h         
saline -6.02 ± 0.15 96.1 ± 3.6 -7.29 ± 0.27 76.4 ± 7.8 -7.82 ± 0.15 98.1 ± 2.2 -8.19 ± 0.17 101.3 ± 0.7
DEP -5.76 ± 0.19 99.3 ± 3.9 -7.51 ± 0.11 88.4 ± 3.6 -8.36 ± 0.05** 98.3 ± 1.4 -8.80 ± 0.24 99.6 ± 1.2
24 h         
saline -6.19 ± 0.11 103.6 ± 5.9 -7.65 ± 0.23 82.0 ± 5.0 -8.40 ± 0.15 99.1 ± 1.1 -8.74 ± 0.16 102.4 ± 0.9
DEP -6.07 ± 0.11 132.9 ± 13.1 -7.58 ± 0.17 80.6 ± 5.8 -8.28 ± 0.19 99.3 ± 3.4 -8.32 ± 0.21 102.5 ± 0.4
  1. Results are mean ± SEM (n = 5-10), obtained by non-linear regression
  2. *P < 0.05, **P < 0.01, control vs DEP, unpaired t-test.
  3. EC50 - concentration producing 50% of maximum contraction
  4. Emax - maximum contraction
  5. IC50 - concentration producing 50% of maximum vasodilatation
  6. Imax - maximum vasodilatation