Figure 2

Thickness of epidermis and dermis, and the number of infiltrating cells on treated skin sites. A. Measurements were performed from H&E-stained skin samples at 100x magnification. The light gray section in the columns represents epidermal and dark grey is the dermal thickness. Statistical significances are indicated as * for epidermal thickness and # for whole skin thickness. **P < 0.01, #P < 0.05. The total inflammatory cells (B), neutrophils (C), eosinophils (D) and mast cells (E) were counted after H&E at 1000x (B-D) and toluidine blue at 400x (E) and expressed as cells per high power field (HPF). The columns and error bars represent means ± SEM (n = 8-16 mice per group). H&E-stained skin of PBS (F), bZnO (G), nZnO (H), OVA/SEB sensitized (I), OVA/SEB and bZnO treated (J), and OVA/SEB and nZnO treated skin (K). Scale bar 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.