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Figure 3 | Particle and Fibre Toxicology

Figure 3

From: Mechanism-based genotoxicity screening of metal oxide nanoparticles using the ToxTracker panel of reporter cell lines

Figure 3

Conventional DNA damage assays confirm the ToxTracker response. (A) Induction of DNA strand breaks by the metal oxide NPs was determined by the comet assay under alkaline conditions. Wild type mES cells were exposed to NPs (20 μg/mL) for 4 h. H2O2 (10 μM for 10 min on ice) was used as positive control. DNA damage was quantified as percentage of DNA in the comet tail. Results are presented as mean ± standerd deviation of 3 independent experiments. (B) Induction of oxidative DNA lesions was determined by FPG comet. Wild type mES cells were exposed to NPs (20 μg/mL) for 4 h and results are expressed as net FPG sites. (C) Induction of γH2AX and RAD51 foci after 4 or 8 h exposure of mES cells to CuO (20 μg/mL), ZnO (30 μg/mL) and NiO (100 μg/mL) NPs as determined by immunocytochemistry. DSBs induction after 10 Gy IR was used as positive control.

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