Figure 8
Hypothalamic inflammatory genes and microglia in response to ICV infusion IMD -0354 in KKay mice exposed to PM 2.5 . A, mRNA for proinflammatory cytokines (TNFα and IL-6) and NF-κB pathway (IKKβ and IκBα) gene expression in the hypothalamus of mice exposed to PM2.5 and treated with an IKKβ inhibitor for 4 weeks. B, Immunohistochemical detection of Iba1 protein, a microglial marker. Representative photomicrographs captured at 20X from KKay mice treated with vehicle or received IKKβ inhibitor (bar = 50 μm). ARC microglia cell number was quantified and normalized to each visual field. C, Representative image (40X) of microglial fine structure in the hypothalamus of vehicle-treated mice exposed to FA and PM2.5 (bar = 50 μm). Mean microglia size was quantified by average number of pixels in 5 representative cells. Data are expressed as mean ± SEM. *P < 0.05 compared to FA-CON group, #P < 0.05 when compared with PM-CON group. n = 7-8 per group.