Figure 1From: Exposure to combustion generated environmentally persistent free radicals enhances severity of influenza virus infectionEffect of EPFR exposure on oxidative stress burden in neonates. A) Neonatal exposure and influenza infection model. Three day old mice were exposed to 200 μg/m3 of DCB230, DCB50, or air for seven consecutive days (0-7 dpe) for 30 min/day (black arrow head) and infected with influenza at 1.25 TCID50/neonate on the fourth dpe (red arrow head). Non-infected lungs were isolated at four dpe for analysis of oxidative stress and regulatory T cells (Tregs). Infected lungs were isolated for pulmonary viral load, flow cytometry, and pulmonary viral clearance on four, six, and eight days post-infection (dpi; asterisks). Pulmonary oxidative stress, determined by levels of (B) 8-isoprostance (IP) and (C) GSH/GSSG ratio in lungs of wild-type C57BL/6 after five days of exposure to air, DCB50 (D50), or DCB230 (D230) and in lungs of hSOD2 transgenic neonates exposed to DCB230 (D230(hSOD+)). Data plotted as mean ± standard error of mean (SEM). *p <0.05 D230 vs Air, D50, and D230(hSOD+); # p <0.05 D230(hSOD+) vs Air, D50, and D230; Brackets indicate p <0.05 D230 vs Air and D230(hSOD+); one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. N =4-10/group.Back to article page