Figure 2

Effect of EPFR exposure on influenza morbidity and viral load in neonates. Neonatal mice were exposed to air, DCB50, or DCB230 and infected i.n. with influenza (AirF, D50F, D230F, D230F^(hSOD+)) at 1.25 TCID₅₀/neonate or sham infected with DPBS (Air). (A) Average percent change in weight plotted against dpi. N =16-35/group. (B) Peak pulmonary viral load assessed at four dpi. N =10-22/group. (C) Pulmonary viral clearance assessed at eight dpi. Dotted line indicates limit of detection (35 TCID₅₀/mL) of assay. (D) Effect of EPFR exposure on influenza infection associated drop in survival in neonates. Survival was recorded daily until 14 dpi. N =8-18/group. Data plotted as mean ± SEM. *p <0.05 D230F vs Air, AirF, D50F, and D230F^(hSOD+). (A) Multiple t tests with Holm-Sidak correction for multiple comparisons. (B and C) One-way ANOVA with Tukey’s multiple comparisons test. (D) Gehan-Breslow Wilcoxon test with Bonferroni correction for multiple comparisons; *p <0.014 between D230F vs AirF and D230F^(hSOD+). N =17-47/group.