Figure 5
From: Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs
Thioredoxin overexpression or pretreatment attenuates the release of IL-1β and HMGB1 in response to cristobalite silica. ELISAs were performed on concentrated medium of BEAS-2B cells transiently overexpressing flagTRX (top panel) or pre-treated with 250 ng/ml recombinant human TRX (lower panel) and exposed to 150 x 106 μm2/cm2 of cristobalite silica for 24 h for the detection of secreted IL-1β (A and C) and HMGB1 (B and D). Data are presented as mean ± SEM with * p-value <0.05 compared to UC, and # p-value <0.05 compared to respective crystalline silica exposure in the pcDNA or non-TRX treated group.