NPs-induced toxicological effects. A. DNA damage detected in single isolated cardiomyocytes by Comet assay (pH >13) in CTRL (white columns) and NPC (red columns) after 1 h and 5 h of exposure. DNA damage is expressed as tail intensity (TI%; *p < 0.05). B. Percent increase of ROS in single isolated cardiomyocytes, NPC (red column) after 1 h. C. Evaluation of TBARS in trachea, lungs and heart tissue after tracheal instillation of saline solution (Vehicle) or saline solution containing TiO2-NPs (2 mg/Kg, NPR). *, p < 0.05 vs. Vehicle.