Figure 2

Pluronic F68 suspended SWCNTs are not cytotoxic to small airway epithelial cells. Cells were exposed to SG65, SG76 or CG200 SWCNTs at doses ranging from 12.5 μg/mL – 100 μg/mL. (A) Cytotoxicity was determined after 24 h of exposure using a trypan blue exclusion assay. Data is presented as the mean % cell viability for each treatment and represents 3 experiments combined. No statistical differences in cytotoxicity were observed for any of the does tested as determined by ANOVA (P < 0.05). Live cells are represented by gray bars and dead cells by white bars. Characterization of SWCNTs in cell media conditions. Each of the SWCNT stocks were incubated with cell media used for exposures and (B) Size distribution was measured for 24 h using dynamic light scattering (DLS). (C) Fractal dimension of the 3 types of SWCNTs were determined in cell culture conditions for 24 h using static light scattering (SLS).