Figure 3

Altered morphology and increased infectivity of SAEC infected with IAV (low dose: MOI-0.1 and high dose: MOI-0.5) with or without pre-treatment of 50 μg/ml SG65 SWCNTs for 24 h. The panels above (A-D) are phase contrast images (200X) of SAEC cells exposed to (A) control pluronic, (B) SWCNTs, (C) IAV and (D) SWCNTs + IAV showing morphological differences between the treatments. (E) Positive immunofluorescence staining (green) of SAEC infected with IAV with or without pre-treatment of SG65 SWCNTs for 24 h. (F) The percent of SAEC infected with IAV is presented as the calculated mean ± standard error of 3 separate experiments. The results show that the number of infected cells increase when pretreated with SG65 SWCNTs for 24 h as determined by ANOVA. *P < 0.05.