Figure 4

Mapping of SWCNTs and IAV in SAEC using darkfield hyperspectral imaging microscopy. Cells were exposed to (B) 50 μg/ml SWCNTs, (C) 5.0 MOI IAV, or (D) SWCNTs + IAV and fixed. (A) representative spectral profile for SWCNTs (blue) and IAV (red). Each subsequent panel shows an image of a cell with mapped locations of SWCNTs (blue) and IAV (red) for each condition. For the single exposures, the images show the (B) presence of SWCNTs in what appears to be aggregates (C) whereas the IAV is present at the cell surface which likely represents budding. (D) In the co-treatment the increased localization of the IAV at the cellular edges and within or on the cells is consistent with the increase in titer observed. The SWCNTs also appear to localize in a different pattern compared to the SWCNT treatment only.