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Figure 4 | Particle and Fibre Toxicology

Figure 4

From: The alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles

Figure 4

IL-1α and IL-1β inhibition reduces neutrophilic inflammation in lung of silica-treated mice. (a) Number of alveolar total cells in WT, IL-1α KO or IL-1β KO mice 24 hours after instillation of silica (crystalline DQ12, 2.5 mg) or not (control). (b) Number of alveolar neutrophils (GR1+ cells) assessed by flow cytometry and (c) expression of the pulmonary neutrophilic CXCR2 marker quantified by qRT-PCR in WT, IL-1α KO or IL-1β KO mice 24 hours after instillation of silica or not. Number of alveolar (d) total cells and (e) neutrophils in WT mice treated with anti-IL-1α or IL-1β neutralizing antibodies or not 18 hours after silica or not. (f) Hematoxylin and eosin-stained lung sections obtained from untreated WT mice or after silica instillation of WT, IL-1α KO and IL-1β KO mice or from untreated mice or after silica instillation of mice injected or not with anti-IL-1α or anti-IL-1β antibodies. Scale bars = 200 μm (large panels) and 100 μm (inserts). Values are means ± SEM of 3 to 5 animals. **p < 0.01 and ***p < 0.001 denote significant difference between animals treated with silica or not. # p < 0.05, ## p < 0.01 and ### p < 0.001 denote significant difference between silica-treated animals with decreased or unmodified IL-1α or IL-1β activity. $$ p < 0.01 denote significant difference in values between IL-1α KO and IL-1β KO silica-treated animals. Ns, denotes no significant difference between silica-treated animals with decreased or unmodified IL-1α or IL-1β activity. P-values are estimated by t-test.

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